SS 1-STEP Polymer HRP + DAB

Catalog Number
QD620-YIKE
(500 tests)
Qty:
QD630-XAKE
(1000 tests)
Qty:
SDS
$0.00
0
Intended Use: IVD

Super Sensitive™ IHC Detection Systems

Immunohistochemistry is a highly sensitive method that allows the localization of antigen within a cell or a tissue with high resolution. The method is based on the use of a primary antibody that specifically binds to its complementary antigen. The bound antibody may then be visualized by a variety of methods such as colorimetric end points.

BioGenex offers three basic types of IHC Detection Systems:

I. Super SensitiveTM (SS) Polymer-HRP IHC Detection System

This is a novel detection system using a non-biotin polymeric technology that makes use of two major components: Super Enhancer and a Poly-HRP reagent. As the system is not based on the biotin-avidin system, problems associated with endogenous biotin are completely eliminated. The enzyme horseradish peroxidase (HRP) catalyzes the conversion of chromogenic substrates (e.g. DAB, AEC) into colored products facilitating tissue staining.

Features & Benefits

  • High signal to noise ratio without endogenous biotin background
  • Excellent sensitivity for weakly expressed antigens
  • Universal system for rabbit and mouse antibodies
  • Excellent cell penetration ability for intense nuclear, cytoplasmic and membrane antigen staining
  • Enabling higher dilution of antibodies for reduced cost
  • Available in RFID tagged (Xmatrx) or barcode labeled (i6000) vials for automation or in drop bottles for easy to use manual staining.

II. Super SensitiveTM (SS) One-Step Polymer-HRP IHC Detection System

All the benefits of SS Polymer-HRP IHC Detection System mentioned above with an easy and fast 15 minutes staining protocol

III. Super SensitiveTM (SS) Link-Label IHC Detection System

A classic system based on the highly specific and sensitive streptavidin-biotin interaction to detect a bound antibody. These kits include multi-Link – a mix of anti-mouse and anti-rabbit IgGs conjugated to multiple biotin molecules and a Label -Strepavidin conjugated with an enzyme (horseradish peroxidase (HRP) or alkaline phosphatase (AP)). The reaction takes place in following steps:

  1. Cells or tissues are prepared and incubated with an unlabeled primary antibody that will bind to the antigen.
  2. The bound antibody is detected with a LINK (species-specific secondary antibody conjugated to biotin).
  3. The bound secondary antibody is then allowed to react with Streptavidin conjugated with an enzyme (Label). Streptavidin binds extremely strongly and irreversibly to the biotin residues on the secondary antibody resulting in the addition of multiple enzyme to the primary antibody complex.
  4. A substrate is then added and acted upon by the enzyme producing a highly visible precipitate. DAB or AEC substrates are available for HRP Labels while Fast Red, Elegance Red and New Fuchsin substrates are available for AP conjugated labels.

Features & Benefits

  • Improved staining of weak antigens
  • User-friendly and extensive choice of kit formats designed for use with human, animal or rodent tissue
  • Wide choice of chromogens offered
  • Available in RFID tagged (Xmatrx) or Barcode labeled (i6000) vials for automation or in drop bottles for easy to use manual staining.

Super Sensitive™ IHC Detection System kits Composition

 
     For manual use (drop bottles)
 
     For i6000 Automation (Barcode labeled)
 
     For Xmatrx Automation (Elite- RFID tagged vials)


Detection Systems- LINK LABEL (For mouse & rabbit antibodies)

SKU Size Multi-Link Label DAB buffer DAB Chromogen Peroxide block Power block Hematox. Negative ctr IgG
QA900-9LE 500 test 50 ml AP 50 ml            
QP900-9LE 500 test 50 ml HRP 50 ml            
QP300-XAKE 1000 100 ml HRP 100 ml            
LP000-ULE 1000+ 5 ml (Conc.) HRP 5 ml (Conc.)            
LA000-ULE 1000+ 5 ml(Conc.) AP 5 ml (Conc.)            
QD470-YCXE
i6000
200 test 20 ml HRP 20 ml 5x20 ml 4 ml 20 ml 20 ml 20 ml  


Detection Systems- - SS Polymer HRP (For mouse & rabbit antibodies)

SKU Size Super enhancer Polymer-HRP DAB buffer DAB Chromogen Peroxide block Power block Hematox. EZ-AR Eleg. 1,2 & 3 Sol. Negative ctr IgG
QD400-60KE 60 test 6 ml 6 ml 10 ml 2 ml 6 ml 6 ml 6 ml   3 ml-Rabbit
3 ml-Mouse
QD420-YIKE 500 test 50 ml 50 ml 50 ml 5 ml          
QD430-XAKE 1000 test 100 ml 100 ml 100 ml 10 ml          
QD440-XAKE 1000 test 100 ml 100 ml              
QD410-YAXE
i6000
200 test 20 ml 20 ml 5x10 ml 4 ml 20 ml 20 ml 20 ml    
QD550-YAXE
Xmatrx-Infinity
200 test 15 ml 15 ml 5x11 ml 4 ml 4x16 ml 21 ml 4x16 ml 16 ml  
QD550-YCDE
Xmatrx-Elite
200 test 16 ml 16 ml 4x11 ml 4 ml 3x16 ml 3x16 ml 3x16 ml 3x16 ml  


Detection Systems- - SS One-Step Polymer HRP (For mouse & rabbit antibodies)

SKU Size Polymer-HRP DAB buffer DAB Chromogen Peroxide block Power block Hematox. EZ-AR Eleg. 1,2 & 3 Sol.
QD620-YIKE 500 test 50 ml 50 ml 5 ml        
QD630-XAKE 1000 test 100 ml 100 ml 10 ml        
QD610-YAXE
i6000
200 test 16 ml 4x11 ml 4 ml 3x16 ml 3x16 ml 3x16 ml  
QD610-YADE
Xmatrx-Elite
200 test 16 ml 4x11 ml 4 ml 3x16 ml 3x16 ml 3x16 ml 3x16 ml

 

Substrates and Chromogens

BioGenex offers complete Substrate Packs for immunohistochemical staining with alkaline phosphatase and peroxidase labels. The kits are designed to reduce substrate preparation time and minimize exposure to chemical hazards. The chart below summarizes the substrates offered, indicating enzyme and standard mounting media compatibility.

Features & Benefits

  • High Resolution AEC and Liquid DAB
  • Rapid Development Time
  • Ready-to-use Solutions
  • Long-Term Stability

Chromogens / Substrates offered

Chromogen Stain Color Enzyme used Solubility in Alcohol/Xylene Compatible Mounting Media
AEC Brick Red HRP Yes Aqueous or Super Mount
DAB Brown HRP No Aqueous, Super Mount or XMount
Elegance Red Red AP No Aqueous, Super Mount or XMount
Fast Red Red AP Yes Aqueous or Super Mount
New Fuchsin Red AP Yes Aqueous or Super Mount