Nucleic Acid Retrieval Solution 1 (NAR-1) - 

HK873-5K

Catalog Number HK873-5K

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Nucleic Acid Retrieval Solution 1 (NAR-1)

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Nucleic Acid Retrieval Method

BioGenex is the inventor of Nucleic Acid Retrieval enabling technology. This technology is an effective way of unmasking DNA in formalin-fixed, paraffin-embedded tissue sections using microwave heating. The Nucleic Acid Retrieval technique breaks the formalin induced cross-linking bonds between DNA and proteins, as well as protein-protein cross-linking thereby allowing better penetration of probes and accessibility of DNA for binding. Nucleic Acid Retrieval (NAR-1) is recommended instead of Proteinase K when DNA targeting probes are used.

Advantages of the method:

  • Reduces time for probe incubation
  • Consistent and reliable staining quality
  • Eliminates false-negative staining results
  • Easy to use - Can be used in both microwave or Xmatrx Automation protocols
  • Non-hazardous, non-flammable and odorless – Safe and Eco-friendly.
Product Method Features & Recommentded Use
NAR-1 Microwave, 95-100°C PH~6, excellent for DNA targeting probes

 

Enzymes for Tissue Digestion

Some tissues require the use of enzymatic pre-treatment before staining to achieve standardized results depending on the antibodies and their different incubation and pre-treatment requirements. Each kit contains three or four vials of lyophilized enzyme powder and 15 ml of reconstitution buffer, enabling you to make fresh enzyme solutions as needed.

  1. Proteinase K in a ready to use (RTU), RNase-free solution and is recommended for use with RNA targeting probes.
  2. The Trypsin and Pepsin kits contain well-established enzymes suitable for routine pre-treatment at 37°C. Pepsin is recommended as pretreatment for FISH applications.
  3. Protease XXIV kits contain a universal digestive agent that allows for fast and effective pre-treatment at room temperature.

 

De-Waxing Solutions

BioGenex deparaffinization solutions are “one-step” products that simultaneously enable the removal of paraffin and allow rehydration of the tissue with a single reagent. In the past, formalin-fixed, paraffin-embedded tissue sections were traditionally deparaffinized with highly toxic, noxious chemicals (i.e. xylene, equivalents). BioGenex, a pioneer in the immunohistochemistry technology, offers xylene-free products that remove the paraffin from mounted tissue slides easily and rapidly.

    1. EZ-DeWax Solution for all BioGenex manual methods.
    2. X-Dewax Solution optimized for Xmatrx automation.

 

    Features & Benefits:
    • Effectively removes paraffin and allows rehydration of the tissue in one step
    • Reduces deparaffinization time from 45 minutes to 10 minutes
    • Eliminates use of toxic solvents (xylene) and minimizes hazardous waste
    • Ready to use (RTU) or 2x solutions (to be diluted 1:1 with ethanol) are available.
  1. EZ-AR Common Solution for microwave facilitated deparaffinization.

 

  • Conveniently performs deparaffinization and Antigen Retrieval in the same slide tank using microwave heating.
  • Quick deparaffinization & rehydration in one step (10 minutes @ 70oC default protocol).
  • Reduces the use of alcohol in preparing tissue sections for IHC, ISH, H & E, FISH and Special Stains.
  • Eliminates use of toxic solvents (xylene) and minimizes hazardous waste.
  • Optimized for use in EZ-Retriever microwave with BioGenex EZ-AR 1-4 solutions.