Double Staining

BioGenex Double Staining IHC products include pre-optimized antibody cocktails and Super Sensitive multiple detection systems, enabling simultaneous testing of multiple antigens on single slide with a fast and easy protocol, assisting rapid and accurate diagnosis.

 

Super SensitiveTM (SS) Double Staining Polymer Detection System

This double staining system is designed with novel polymer technology for fast and easy IHC staining of multiple antigens on a single slide. This system is pre-optimized for human tissues with superior sensitivity and specificity to produce precise and reliable results that allow easy interpretation and accurate diagnosis.

    Features & Benefits
  • Pre-mixed and pre-optimized polymer cocktails
  • Easy and fast staining with a 4-step protocol
  • Reduced costs by maximizing resources
  • Clean and intense stain without endogenous biotin background
  • Excellent sensitivity for weakly expressed antigens
  • Excellent cell penetration for intense nuclear, cytoplasmic and membrane antigen staining
  • Enables higher dilution of antibodies for reduced cost
  • Available in RFID tagged vials for Xmatrx automation or in drop bottles for easy to use manual staining.
SKU Size Anti-mouse Polymer-X Anti-rabbit Polymer-Y DAB buffer DAB Chromo. Peroxide block Power block Fast Red Hematox Negative ctrl IgG EZ-AR Eleg. 1,2 & 3 Sol.
QS400-60KE 60 test 6 ml HRP 6 ml AP 10 ml 4 ml 12 ml 12 ml 20 ml A
20 ml B
  6 ml Rb
6 ml Mo
 
QS200-60KE 60 test 6 ml AP 6 ml HRP 10 ml 4 ml 12 ml 12 ml 20 ml A
20 ml B
  6 ml Rb
6 ml Mo
 
QS410-YIKE 500 test 50 ml HRP 50 ml AP                
QS210-YIKE 500 test 50 ml AP 50 ml HRP                
QS400-YADE
Xmatrx-Elite
100 test 7 ml HRP 7 ml AP 4 x 5 ml 3 ml 2x10 ml 2x10 ml 2x14 ml A
2x14 ml B
3x10 ml 7 ml Rb
7 ml Mo
3x7 ml
QS200-YADE
Xmatrx-Elite
100 test 7 ml AP 7 ml HRP 4 x 5 ml 3 ml 2x10 ml 2x10 ml 2x14 ml A
2x14 ml B
3x10 ml 7 ml Rb
7 ml Mo
3x7 ml

Substrates and Chromogens

BioGenex offers complete Substrate Packs for immunohistochemical staining with alkaline phosphatase and peroxidase labels. The kits are designed to reduce substrate preparation time and minimize exposure to chemical hazards. The chart below summarizes the substrates offered, indicating enzyme and standard mounting media compatibility.

Features & Benefits

  • High Resolution AEC and Liquid DAB
  • Rapid Development Time
  • Ready-to-use Solutions
  • Long-Term Stability

The chart below summarizes the compatibility of mounting medium, chromogens and counterstains.

Chromogen Stain Color Enzyme used Solubility in Alcohol/Xylene Compatible with Hematoxylin Compatible Mounting Media
AEC Brick Red HRP Yes Yes Aqueous or Super Mount
DAB Brown HRP No Yes Aqueous, Super Mount or XMount
Elegance Red Red AP No Yes Aqueous, Super Mount or XMount
Fast Red Red AP Yes Yes Aqueous or Super Mount
New Fuchsin Red AP Yes Yes Aqueous or Super Mount