EZ-AR™ 1 Elegance, EZ-AR™ 2 Elegance, EZ-AR™ 3 Elegance, and EZ-AR™ 4 Elegance Solutions(HX031-YCD, HX032-YCD, HX033-YCD, and HX034-YCD):
These solutions perform antigen retrieval in formalin-fixed, paraffin-embedded tissue sections using heat retrieval. Different tissues require different pretreatment conditions. Depending on the nature of the tissue and the antigen retrieval requirement, one of the EZ-AR™ Elegance solutions may be used. EZ-ARä 1 Elegance and EZ-ARä 3 Elegance are Citra-based solutions, EZ-ARä 2 Elegance is Tris based solution. The use of EZ-AR™ 1 Elegance, EZ-AR™ 2 Elegance, EZ-AR™ 3 Elegance or EZ-AR™ 4 Elegance in combination with XDeWax™ ensures uniform dewaxing, rehydration, and antigen retrieval.
The main advantages that these solutions offer are:
- Eliminates the use of enzymes as a pretreatment in most cases, hence removing the guesswork from optimizing treatment time on the basis of tissue fixation.
- Reduces background staining: EZ-AR™ Elegance solutions increase the availability of antigenic epitopes in tissues due to their capability of being heated up to a temperature of 110°C without boiling. One may need to use Avidin/Biotin blocks to block endogenous biotin when using Biotin-Streptavidin based detection systems.
- Reduces incubation time with primary antibodies
- Better quality staining due to the unique properties of the solution.
- Preserves morphology of tissues.
- Non-toxic and non-flammable.